TitleEngineering of a Histone-Recognition Domain in Dnmt3a Alters the Epigenetic Landscape and Phenotypic Features of Mouse ESCs.
Publication TypeJournal Article
Year of Publication2015
AuthorsNoh, Kyung-Min, Wang Haibo, Kim Hyunjae R., Wenderski Wendy, Fang Fang, Li Charles H., Dewell Scott, Hughes Stephen H., Melnick Ari M., Patel Dinshaw J., Li Haitao, and C Allis David
JournalMol Cell
Date Published2015 Jul 02
KeywordsAnimals, Cell Differentiation, DNA (Cytosine-5-)-Methyltransferases, DNA Helicases, DNA Methylation, DNA Methyltransferase 3A, Embryonic Stem Cells, Histones, Mice, Mice, Inbred C57BL, Mitosis, Nuclear Proteins, Phosphorylation, Promoter Regions, Genetic, Protein Engineering, Protein Structure, Tertiary, X-linked Nuclear Protein

<p>Histone modification and DNA methylation are associated with varying epigenetic "landscapes," but detailed mechanistic and functional links between the two remain unclear. Using the ATRX-DNMT3-DNMT3L (ADD) domain of the DNA methyltransferase Dnmt3a as a paradigm, we apply protein engineering to dissect the molecular interactions underlying the recruitment of this enzyme to specific regions of chromatin in mouse embryonic stem cells (ESCs). By rendering the ADD domain insensitive to histone modification, specifically H3K4 methylation or H3T3 phosphorylation, we demonstrate the consequence of dysregulated Dnmt3a binding and activity. Targeting of a Dnmt3a mutant to H3K4me3 promoters decreases gene expression in a subset of developmental genes and alters ESC differentiation, whereas aberrant binding of another mutant to H3T3ph during mitosis promotes chromosome instability. Our studies support the general view that histone modification "reading" and DNA methylation are closely coupled in mammalian cells, and suggest an avenue for the functional assessment of chromatin-associated proteins.</p>

Alternate JournalMol Cell
PubMed ID26073541
PubMed Central IDPMC4491196
Grant ListP30 CA008748 / CA / NCI NIH HHS / United States
P50 MH096890 / MH / NIMH NIH HHS / United States
R01 MH094698 / MH / NIMH NIH HHS / United States
MH 094698 / MH / NIMH NIH HHS / United States