TitleMicroscale Bioadhesive Hydrogel Arrays for Cell Engineering Applications.
Publication TypeJournal Article
Year of Publication2014
AuthorsPatel, Ravi Ghanshyam, Purwada Alberto, Cerchietti Leandro, Inghirami Giorgio, Melnick Ari, Gaharwar Akhilesh K., and Singh Ankur
JournalCell Mol Bioeng
Volume7
Issue3
Pagination394-408
Date Published2014 Sep 01
ISSN1865-5025
Abstract

<p>Bioengineered hydrogels have been explored in cell and tissue engineering applications to support cell growth and modulate its behavior. A rationally designed scaffold should allow for encapsulated cells to survive, adhere, proliferate, remodel the niche, and can be used for controlled delivery of biomolecules. Here we report a microarray of composite bioadhesive microgels with modular dimensions, tunable mechanical properties and bulk modified adhesive biomolecule composition. Composite bioadhesive microgels of maleimide functionalized polyethylene glycol (PEG-MAL) with interpenetrating network (IPN) of gelatin ionically cross-linked with silicate nanoparticles were engineered by integrating microfabrication with Michael-type addition chemistry and ionic gelation. By encapsulating clinically relevant anchorage-dependent cervical cancer cells and suspension leukemia cells as cell culture models in these composite microgels, we demonstrate enhanced cell spreading, survival, and metabolic activity compared to control gels. The composite bioadhesive hydrogels represent a platform that could be used to study independent effect of stiffness and adhesive ligand density on cell survival and function. We envision that such microarrays of cell adhesive microenvironments, which do not require harsh chemical and UV crosslinking conditions, will provide a more efficacious cell culture platform that can be used to study cell behavior and survival, function as building blocks to fabricate 3D tissue structures, cell delivery systems, and high throughput drug screening devices.</p>

DOI10.1007/s12195-014-0353-8
Alternate JournalCell Mol Bioeng
PubMed ID25328548
PubMed Central IDPMC4196273
Grant ListR21 CA185236 / CA / NCI NIH HHS / United States